| 质粒名称: | pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 69906 |
| 存储实验室: | Jeremy Wilusz |
|
载体骨架基本信息 |
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| 载体名称: | pcDNA3.1(+) ZKSCAN1 MCS Exon Vector |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6705 bp |
| 完整质粒大小: | 8151 bp |
| 载体类型: | 哺乳细胞表达 |
| 生长条件: | 37 ℃ |
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插入基因信息 |
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| 插入基因名称: | ciRS7,CDR1as |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1485 bp |
| 启动子 | CMV |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRV(未破坏) |
| 3' 克隆位点: | SacII(未破坏) |
| 5' 测序引物 | CGCAAATGGGCGGTAGGCGTG |
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文献引用方法 |
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| 材料和方法部分: | pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon was a gift from Jeremy Wilusz (Addgene plasmid # 69906 ; http://n2t.net/addgene:69906 ; RRID:Addgene_69906) |
| 参考文献部分: | Combinatorial control of Drosophila circular RNA expression by intronic repeats, hnRNPs, and SR proteins. Kramer MC, Liang D, Tatomer DC, Gold B, March ZM, Cherry S, Wilusz JE. Genes Dev. 2015 Oct 8. 10.1101/gad.270421.115 PubMed 26450910 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
 ZKSCAN1 MCS-ciRS7 Exon质粒图谱.png)
载体简介
载体序列
LOCUS pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon 8150 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Expresses the human ciRS7/CDR1as circular RNA in mammalian cells. ACCESSION . VERSION . KEYWORDS pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8150) AUTHORS Kramer MC, Liang D, Tatomer DC, Gold B, March ZM, Cherry S, Wilusz JE TITLE Combinatorial control of Drosophila circular RNA expression by intronic repeats, hnRNPs, and SR proteins. JOURNAL Genes Dev. 2015 Oct 8. PUBMED 26450910 REFERENCE 2 (bases 1 to 8150) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8150 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(44..63) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer 235..614 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 615..818 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 769..789 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 863..882 /label=T7 /note="T7 promoter, forward primer" promoter 863..881 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3744..3761) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 3750..3974 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 4020..4448 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(4107..4126) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 4317..4338 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(4457..4477) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4462..4791 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4642..4777 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4704..4723 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 4858..5652 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4912..4931) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5522..5541 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 5826..5947 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(5863..5882) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 5917..5936 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(5996..6012) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(5996..6012) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(6009..6031) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 6020..6036 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(6044..6074) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6089..6110 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(6227..6244) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(6398..6983) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(6478..6497) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7154..8014) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 7777..7796 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8015..8119) /gene="bla" /label=AmpR promoter ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcagt 961 gacagtggag attgtacagt tttttcctcg atttgtcagg attttttttt tttgacggag 1021 tttaacttct tgtctcccag gtaggaagtg cagtggcgta atctcggctc actacaacct 1081 ccacctcctg ggttcaagcg tttctcctgc ctcagctttc cgagtagctg ggattacagg 1141 cgcctgccac catgccctgc tgacttttgt atttttagta gagacggggt ttcaccatgt 1201 tggccaggct ggtcttgaac tcctgaccgc aggcgattgg cctgcctcgg cctcccaaag 1261 tgctgagatt acaggcgtga gccaccaccc ccggcctcag gagcgttctg atagtgcctc 1321 gatgtgctgc ctcctataaa gtgttagcag cacagatcac tttttgtaaa ggtacgtact 1381 aatgactttt tttttatact tcaggatatc ggtttccgat ggcacctgtg tcaaggtctt 1441 ccaacaactc cgggtcttcc agcgacttca agtcttccaa taatctcaag gtcttccaga 1501 taatcctgag cttccagaaa atccacatct tccagacaat ccatgtcttc cggacaatcc 1561 atgtcttcca agaagctcca agtcttccag taaatcaagt cttccagcaa atccagtctt 1621 ccagcaatta ctggtcttcc accaaatcca gatcttccag gaaaatccac gtcttccagg 1681 aaatccatgt cttccaataa tttcaaggtc ttccatcaaa tacagatctt ccagctaatc 1741 catgtcttcc agaaaaatct gtgtcttcca ccaaatccaa gtcttccagt aaatctagtt 1801 cttccagaaa aatctagatc ttccagtcaa tcagtgtctt ccagaaagaa atccaggtct 1861 tccagtcaat cagtgtcttc cagaaagaaa tccaggtctt ccagtcagtc agtgtcttcc 1921 agaaaaatct acgtcttcca ccaaatccag gtcttccagt caatccacat cttccggaaa 1981 aaatccaggt cttccagcca atatatgtct tcctgaagat ccacgtcttc cagaaaatcc 2041 atgtcttcca gaaaatccat gtcttccagt aacctcccag tcttccagaa aatccacgtc 2101 ttcccaacaa tccaagtctt ccggataatt tgggtcttcc tgaaaatcta cgtcttccaa 2161 aaaagccatg tcttccagaa aatccacatc ttccaatggc ctccaggtct tccagactat 2221 ccatgtcttc cagaaaatcc ttgtcttccc ttaaatctat agcttccaaa aaatccgggt 2281 cttccaggaa atccgtgtct tccagcaagt ccacgtcttc caacaaagcc atgtcttcca 2341 gactatccat gtcttccaga aaatccttgt cttccctcaa atccatagct tccgaaaaat 2401 ccaggtcttc caggaaatcc gtgtcttcca gcaaatccac gtcttccaac aaagccatgt 2461 cttccatcaa attaatgtct tccagcctac ttgtgtcttc caacaaaggt acgtcttcca 2521 acaaaggtac gtcttccaac aaaggtatgt cttccaacaa aggtacgtct tccagaaaat 2581 ccacgtcttc caaccaagcc atgtcttcca gaaaatccac gtcttccaga aaatatatgt 2641 cttccaacta agctacgtct tccaacaaat ccatgtcttc ctatatctcc aggtcttcca 2701 gcatctccag ggcttccagc atctgctcgt cttccaacat ctccacgtct tccagcatct 2761 ctgtgtcttc cagcatcttc atgtcttcca acaactaccc agtcttccat caactggctc 2821 aatatccatg tcttccaacg tctccagtgt gctgatcttc tgacattcag gtcttccagt 2881 gtctgcaata tccagccgcg gaggtaagaa gcaaggtttc atttagggga agggaaatga 2941 ttcaggacga gagtctttgt gctgctgagt gcctgtgatg aagaagcatg ttagtcctgg 3001 gcaacgtagc gagaccccat ctctacaaaa aatagaaaaa ttagccaggt atagtggcgc 3061 acacctgtga ttccagctac gcaggaggct gaggtgggag gattgcttga gcccaggagg 3121 ttgaggctgc agtgagctgt aatcatgcca ctactccaac ctgggcaaca cagcaaggac 3181 cctgtctcaa aagctactta cagaaaagaa ttaggctcgg cacggtagct cacacctgta 3241 atcccagcac tttgggaggc tgaggcgggc agatcacttg aggtcaggag tttgagacca 3301 gcctggccaa catggtgaaa ccttgtctct actaaaaata tgaaaattag ccaggcatgg 3361 tggcacattc ctgtaatccc agctactcgg gaggctgagg caggagaatc acttgaaccc 3421 aggaggtgga ggttgcagta agccgagatc gtaccactgt gctctagcct tggtgacaga 3481 gcgagactgt cttaaaaaaa aaaaaaaaaa aaaaagaatt aattaaaaat ttaaaaaaaa 3541 atgaaaaaaa gctgcatgct tgttttttgt ttttagttat tctacattgt tgtcattatt 3601 accaaatatt ggggaaaata caacttacag accaatctca ggagttaaat gttactacga 3661 aggcaaatga actatgcgta atgaacctgg taggcattag cggccgctcg agtctagagg 3721 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 3781 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 3841 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 3901 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 3961 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 4021 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 4081 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 4141 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 4201 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 4261 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 4321 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 4381 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 4441 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 4501 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 4561 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 4621 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 4681 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag 4741 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg 4801 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg 4861 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc 4921 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg 4981 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag 5041 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc 5101 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat 5161 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg 5221 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc 5281 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag 5341 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc 5401 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc 5461 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata 5521 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc 5581 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac 5641 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc 5701 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt 5761 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc 5821 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 5881 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 5941 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat 6001 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 6061 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 6121 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 6181 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 6241 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 6301 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6361 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 6421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 6481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 6541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 6601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 6661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 6721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 6781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 6841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 6901 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta 6961 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 7021 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 7081 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 7141 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 7201 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 7261 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 7321 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 7381 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 7441 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 7501 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 7561 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 7621 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 7681 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 7741 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 7801 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 7861 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 7921 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 7981 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 8041 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 8101 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc //
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